Charlotte of Cyprus or Charlotte de Lusignan (Nicosia, 28 June 1444 - Rome, 16 July 1487), was the Queen of Cyprus, Titular Queen of Jerusalem and Armenia, and the suo jure Princess of Antioch. She was the eldest and only surviving daughter of King John II of Cyprus and Helena Palaiologina. At the age of 14, she succeeded to the Cypriot throne upon the death of her father in 1458. Her illegitimate half-brother, James challenged her right to the crown. With the support of the Egyptians, he forced her to flee the island in 1463, and he was later crowned king. She made a military attempt to regain her throne, but was unsuccessful, and died childless in Rome.

Queen of Cyprus

Charlotte's paternal grandparents were King Janus of Cyprus and Charlotte de Bourbon-La Marche.

Her mother was a daughter of Theodore II Palaiologos, Lord of Morea and his wife Cleope Malatesta. Theodore was in turn a son of Eastern Roman Emperor Manuel II Palaeologus and Helena Dragaš.

Charlotte succeeded as Princess of Antioch in 1456. At the age of 14, upon her father's death, Charlotte became Queen of Cyprus, ruling from 1458 to 1460 or 1464. Her right to the throne was challenged by her illegitimate half-brother James who captured Famagusta and Nicosia with Egyptian support. After being blockaded in the castle of Kyrenia for three years, in 1463 she fled to Rome, whereupon her half-brother was crowned King James II.

She made an unsuccessful military attempt to regain her throne with Papal support.

Marriages

Charlotte married twice:

• Infante John of Portugal, also known as John of Coimbra, (1431 or 1433 – between July and 11 September 1457) (son of Infante Pedro, Duke of Coimbra and grandson of King John I of Portugal), in Nicosia, May, 1456. He was made a Titular Prince of Antioch. It is rumoured that his death was a murder due to poisoning, instigated by his mother-in-law Queen Helena, who arranged Charlotte's second marriage.

• Ludovico di Savoia, Count of Geneva (Geneva, 5 June 1436 or 1 April 1437 – Château-Monastery de Ripaille, August, 1482). This couple were married in 4 October 1459. Louis had married firstly in 1 April/14 December 1447 Annabella of Scotland (ca 1433 – after 1471), but this marriage was annulled in 1458, and she later married and divorced George Gordon, 2nd Earl of Huntly. Louis was the second son and namesake of Louis, Count of Savoy by Anne de Lusignan, daughter of Janus of Cyprus and became a Consort King of Cyprus from 1459 to 1462 and also a Titular Consort King of Jerusalem.

By her second husband Louis, Charlotte had an unnamed son who was born in July 1464, but the boy died within a month of his birth.

She died childless on 16 July 1487, shortly after her forty-third birthday. She adopted as her son, Alfonso of Aragon, the illegitimate son of King Ferrante II of Naples, who was married to her half-brother's illegitimate daughter, Charlotte de Lusignan. However, in February 1485, in exchange for an annual pension of 4,300 florins, she instead ceded her claims to her nephew Charles I of Savoy, the next in the legitimate line of succession.

Charlotte is buried in St. Peter's Basilica.