Beatrice of Castile (8 March 1293 – 25 October 1359) was Queen of Portugal by marriage and Infanta of Castile-León by birth. She was the wife of King Afonso IV of Portugal, and the youngest daughter of King Sancho IV of Castile and his Queen, María de Molina.

On 12 September 1309, Beatrice was married to the later Afonso IV of Portugal who was the only son of Denis of Portugal and Elizabeth of Aragon. They had the following children: